diff --git a/bitcoin/signature.py b/bitcoin/signature.py
index 89434b7a..1a0532dc 100644
--- a/bitcoin/signature.py
+++ b/bitcoin/signature.py
@@ -38,11 +38,13 @@ def stream_deserialize(cls, f):
         return cls(r, s, len(r + s))
 
     def stream_serialize(self, f):
+        rs = BytesIO()
+        rs.write(b"\x02")
+        BytesSerializer.stream_serialize(self.r, rs)
+        rs.write(b"\x02")
+        BytesSerializer.stream_serialize(self.s, rs)
         f.write(b"\x30")
-        f.write(b"\x02")
-        BytesSerializer.stream_serialize(self.r, f)
-        f.write(b"\x30")
-        BytesSerializer.stream_serialize(self.s, f)
+        BytesSerializer.stream_serialize(rs.getvalue(), f)
 
     def __repr__(self):
         return 'DERSignature(%s, %s)' % (self.r, self.s)
diff --git a/bitcoin/tests/test_signature.py b/bitcoin/tests/test_signature.py
new file mode 100644
index 00000000..b64624ac
--- /dev/null
+++ b/bitcoin/tests/test_signature.py
@@ -0,0 +1,42 @@
+# Copyright (C) The python-bitcoinlib developers
+# This file is subject to the license terms in the LICENSE file.
+
+import unittest
+from io import BytesIO
+
+from bitcoin.core.key import CECKey
+from bitcoin.signature import DERSignature
+
+
+class Test_DERSignature(unittest.TestCase):
+    def test_serialize_known_vectors(self):
+        # ECDSA integers can require a leading zero to preserve their sign.
+        for r, s in [(b'\x01', b'\x01'),
+                     (b'\x7f' * 32, b'\x01'),
+                     (b'\x00\x80' + b'\x00' * 31, b'\x7f' * 32),
+                     (b'\x00\xff' + b'\x00' * 31,
+                      b'\x00\x80' + b'\x00' * 31)]:
+            with self.subTest(r=r, s=s):
+                body = b'\x02' + bytes([len(r)]) + r
+                body += b'\x02' + bytes([len(s)]) + s
+                expected = b'\x30' + bytes([len(body)]) + body
+                signature = DERSignature.deserialize(expected)
+                self.assertEqual(signature.serialize(), expected)
+                restored = DERSignature.deserialize(signature.serialize())
+                self.assertEqual((restored.r, restored.s), (r, s))
+
+    def test_stream_serialize(self):
+        stream = BytesIO()
+        signature = DERSignature(b'\x01', b'\x02', 2)
+        signature.stream_serialize(stream)
+        self.assertEqual(stream.getvalue(), bytes.fromhex('3006020101020102'))
+
+    def test_generated_signatures_verify_after_roundtrip(self):
+        key = CECKey()
+        key.set_secretbytes(b'\x01' * 32)
+        for digest in (b'\x02' * 32, b'\x03' * 32, b'\xff' * 32):
+            with self.subTest(digest=digest):
+                encoded = key.sign(digest)
+                restored = DERSignature.deserialize(encoded).serialize()
+                self.assertEqual(restored, encoded)
+                self.assertTrue(key.verify(digest, restored))